EXPRESSION OF PETASE ENZYME IN ESCHERICHIA COLI BACTERIA FOR EFFICIENT DEGRADATION OF POLYETHYLENE
Keywords:
деградация пластика ПЭТ, Ideonella sakaiensis, ПЭТаза, ферментная инженерия, сигнальный пептид.
Abstract
Plastic pollution, primarily associated with polyethylene terephthalate (PET) plastics, has become a major global environmental crisis. Ideonella sakaiensis strain 201-F6 is the most effective solution to degrade PET. In this article, we described the results of studies on cloning the FAST-PETase enzyme with thermostable mutations and its expression in E.coli bacteria, purifying the protein by nickel chelate chromatography, and determining the enzyme activity. The study results showed that the active FAST-PETase enzyme was effectively purified.
References
1. Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, Toyohara K, Miyamoto K, Kimura Y, Oda K. A bacterium that degrades and assimilates poly(ethylene terephthalate), Science, vol. 351, no. 6278, pp. 1196–1199, Mar. 2016, doi: 10.1126/science.aad6359.
2. Han X, Liu W, Huang JW, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT. Structural insight into catalytic mechanism of PET hydrolase, Nat. Commun., vol. 8, no. 1, p. 2106, Dec. 2017, doi: 10.1038/s41467-017-02255-z.
3. Liu C, Shi C, Zhu S, Wei R, Yin CC. Structural and functional characterization of polyethylene terephthalate hydrolase from Ideonella sakaiensis, Biochem. Biophys. Res. Commun., vol. 508, no. 1, pp. 289–294, Jan. 2019, doi: 10.1016/j.bbrc.2018.11.148.
4. Shi L, Liu H, Gao S, Weng Y, Zhu L. Enhanced Extracellular Production of Is PETase in Escherichia coli via Engineering of the pelB Signal Peptide, J. Agric. Food Chem., vol. 69, no. 7, pp. 2245–2252, Feb. 2021, doi: 10.1021/acs.jafc.0c07469.
5. Son HF, Joo S, Seo H, Sagong HY, Lee SH, Hong H, Kim KJ. Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis, Enzyme Microb. Technol., vol. 141, p. 109656, Nov. 2020, doi: 10.1016/j.enzmictec.2020.109656.
6. Yinglu C, Yanchun Ch, Xinyue L, Saijun D, Yu’ e T, Yuxin Q, Ruchira M, Jing H, Chunli L, Xu H, Weidong L, Quan Ch, Wangqing W, Xin W, Wenbin D, Shuangyan T, Hua X, Haiyan L, Yong L, Kendall N, Bian W. Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS Catal., vol. 11, no. 3, pp. 1340–1350, Feb. 2021, doi: 10.1021/acscatal.0c05126.
7. Lu H, Diaz DJ, Czarnecki NJ, Zhu C, Kim W, Shroff R, Acosta DJ, Alexander BR, Cole HO, Zhang Y, Lynd NA, Ellington AD, Alper HS. Machine learning-aided engineering of hydrolases for PET depolymerization, Nature, vol. 604, no. 7907, pp. 662–667, Apr. 2022, doi: 10.1038/s41586-022-04599-z.
8. https://www.neb.com/en/protocols/0001/01/01/protocol-for-expression-using-neb-express-2523.
2. Han X, Liu W, Huang JW, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT. Structural insight into catalytic mechanism of PET hydrolase, Nat. Commun., vol. 8, no. 1, p. 2106, Dec. 2017, doi: 10.1038/s41467-017-02255-z.
3. Liu C, Shi C, Zhu S, Wei R, Yin CC. Structural and functional characterization of polyethylene terephthalate hydrolase from Ideonella sakaiensis, Biochem. Biophys. Res. Commun., vol. 508, no. 1, pp. 289–294, Jan. 2019, doi: 10.1016/j.bbrc.2018.11.148.
4. Shi L, Liu H, Gao S, Weng Y, Zhu L. Enhanced Extracellular Production of Is PETase in Escherichia coli via Engineering of the pelB Signal Peptide, J. Agric. Food Chem., vol. 69, no. 7, pp. 2245–2252, Feb. 2021, doi: 10.1021/acs.jafc.0c07469.
5. Son HF, Joo S, Seo H, Sagong HY, Lee SH, Hong H, Kim KJ. Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis, Enzyme Microb. Technol., vol. 141, p. 109656, Nov. 2020, doi: 10.1016/j.enzmictec.2020.109656.
6. Yinglu C, Yanchun Ch, Xinyue L, Saijun D, Yu’ e T, Yuxin Q, Ruchira M, Jing H, Chunli L, Xu H, Weidong L, Quan Ch, Wangqing W, Xin W, Wenbin D, Shuangyan T, Hua X, Haiyan L, Yong L, Kendall N, Bian W. Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS Catal., vol. 11, no. 3, pp. 1340–1350, Feb. 2021, doi: 10.1021/acscatal.0c05126.
7. Lu H, Diaz DJ, Czarnecki NJ, Zhu C, Kim W, Shroff R, Acosta DJ, Alexander BR, Cole HO, Zhang Y, Lynd NA, Ellington AD, Alper HS. Machine learning-aided engineering of hydrolases for PET depolymerization, Nature, vol. 604, no. 7907, pp. 662–667, Apr. 2022, doi: 10.1038/s41586-022-04599-z.
8. https://www.neb.com/en/protocols/0001/01/01/protocol-for-expression-using-neb-express-2523.
Published
2024-03-30
How to Cite
Muhammad Latif NAZIROV, Ilxom XALILOV, & Fazliddin QOBILOV,. (2024). EXPRESSION OF PETASE ENZYME IN ESCHERICHIA COLI BACTERIA FOR EFFICIENT DEGRADATION OF POLYETHYLENE. News of the NUUz, 3(3.1), 81-85. https://doi.org/10.69617/uzmu.v3i3.1.1718
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