OBTAINING A RECOMBINANT TYPE OF PLANT DEFENSIN WITH ANTIMICROBIAL AND FUNGICIDAL ACTIVITY
Keywords:
defensins, Nigella sativa, Ns-D2, recombinant DNA, vector.
Abstract
O’simliklar tarkibida mikroorganizmlar membranasiga ta’sir etib, ularni parchalaydigan oqsil va peptidlar mavjud. Ushbu birikmalar antibiotiklarga qaraganda samaraliroq va patogenlarga chidamli transgen organizmlarni yaratish uchun qo’llanilishi mumkin. Biroq, mikroblarga qarshi peptidlarni tabiiy ob'ektlardan izlash va ajratib olish murakkab va uzoq vaqt talab etadigan jarayondir. Bizning tadqiqotlarimiz natijasida o'simlikdan olingan peptidlarning faolligi o'rganildi, Ns-D2 defensin geni bilan rekombinant DNK tuzildi va rekombinant defensinning antimikrob va fungitsid faolligi tasdiqlandi. Shunday qilib, defenzin olish uchun muqobil va qulayroq usul taklif qilindi.
References
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2. Broekaert WF, Terras FRG, Cammue BPA, Vanderleyden J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiology Letters. 1990;69:55–9.
3. Mee Do H, Chul Lee S, Won Jung H, Hoon Sohn K, Kook Hwang B. Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum. Plant Science. 2004;166:1297–305.
4. Huang HW. Action of antimicrobial peptides: two-state model. Biochemistry. 2000;39:8347–52.
5. Mosolov VV, Valueva TA. [Proteinase inhibitors and their function in plants: a review]. Prikl Biokhim Mikrobiol. 2005;41:261–82.
6. Ganz T. Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol. 2003;3:710–20.
7. Hannan MA, Rahman MA, Sohag AAM, Uddin MJ, Dash R, Sikder MH, et al. Black Cumin (Nigella sativa L.): A Comprehensive Review on Phytochemistry, Health Benefits, Molecular Pharmacology, and Safety. Nutrients. 2021;13:1784.
8. Rogozhin EA, Oshchepkova YI, Odintsova TI, Khadeeva NV, Veshkurova ON, Egorov TA, et al. Novel antifungal defensins from Nigella sativa L. seeds. Plant Physiol Biochem. 2011;49:131–7.
9. Rodrigues da Cunha L, Fortes Ferreira CLL, Durmaz E, Goh YJ, Sanozky-Dawes R, Klaenhammer T. Characterization of Lactobacillus gasseri isolates from a breast-fed infant. Gut Microbes. 2012;3:15–24.
10. Bulet P, Stöcklin R. Insect antimicrobial peptides: structures, properties and gene regulation. Protein Pept Lett. 2005;12:3–11.
11. Chen G-H, Hsu M-P, Tan C-H, Sung H-Y, Kuo CG, Fan M-J, et al. Cloning and characterization of a plant defensin VaD1 from azuki bean. J Agric Food Chem. 2005;53:982–8.
Published
2024-03-29
How to Cite
Владимир ЦОЙ, Дильбар ДАЛИМОВА, Шахноза ИБРАГИМОВА, Музаффар МУМИНОВ, Дарья ЗАКИРОВА, & Шахло ТУРДИКУЛОВА. (2024). OBTAINING A RECOMBINANT TYPE OF PLANT DEFENSIN WITH ANTIMICROBIAL AND FUNGICIDAL ACTIVITY. News of the NUUz, 3(3.1), 162-165. https://doi.org/10.69617/uzmu.v3i3.1.1752
Section
Articles
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