POLIETILENNI SAMARALI PARCHALASH UCHUN PETaza FERMENTINI ESCHERICHIA COLI BAKTERIYASIDA EXSPRESSIYALASH
"Maqola"
Plastik ifloslanish, birinchi navbatda, polietilen tereftalat (PET) plastmassalari bilan bog'liq bo'lib, jiddiy global ekologik inqirozga aylandi. Bakteriyaning Ideonella sakaiensis 201-F6 turi shtami PETni parchalash uchun eng samaralii yechim sifatida foydalaniladi. Biz ushbu maqolada, termostabil mutatsiyalarga ega bo’lgan FAST-PETaza fermentini klonlash va uni E.coli bakteriyasida ekspressiyalash, oqsilni nikel xelat xromotagrafiya usulida tozalab olish va ferment aktivligini aniqlash kabi tadqiqot natijalarini bayon etdik. Tadqiqot natijalari aktivlikga ega bo’lgan FAST-PETaza fermentini samarali tozalab olinganini ko’rsatdi
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