ANTAGONIST AND ANTIBACTERIAL PROPERTIES OF THE BACTERIAL STRAIN BACILLUS AMYLOLIQUEFACIENS –UZMU 22

  • Moxichehra SHOXIDDINOVA O’zMU Biologiya fakulteti Mikrobiologiya va biotexnologiya kafedrasi o'qituvchisi
  • Mujassam AXMEDOVA, O'zMU Biologiya fakulteti Mikrobiologiya va virusologiya mutahasissligi 1-bosqich magistranti
  • Nozima YUSUFDJANOVA O'zMU Biologiya fakulteti 2-bosqich talabasi
  • Behro'z TOJIYEV O'zR FA Mikrobiologiya instituti 1-bosqich Tayanch doktoranti
  • Moxichehra PATTAYEVA, O'zRFA Genetika va o'simliklar eksperimental biologiyasi instituti katta ilmiy hodim, PhD
  • Qunduz NORMURODOVA O’zMU Biologiya fakulteti Mikrobiologiya va biotexnologiya kafedrasi prof.v.b. B.f.d.
Keywords: Kalanxoye degremona dorivor o‘simligining ildizidan 2ta, poyasidan 3ta va bargidan 3ta jami 8ta endofit izolyatlar ajratib olindi. Skrining natijasida Kalanxoye degremonadan ajratib olingan K7 izolyati tanlab olindi va MALDI TOF mass-spektrometriya usuli yordamida identifikatsiya qilindi. Tanlab olingan K7 bakteriya izolyati B.amyloliquefaciens ekanligi aniqlandi va B.amyloliquefaciens – UzMU 22 shtammi deb nomlandi. Bacillus amyloliquefaciens - UzMU 22 shtammining Verticillium dahliae, Alternaria alternata, Fusarium solani ва Fusarium oxysporum kabi patogen zamburug‘larga nisbatan antogonistik faolligi, kanamitsin, eritromitsin, sefotoksin, seprofloksatsin, gentamitsin va lenkomitsin kabi bir nechta antibiotiklarga sezgirligi va gidrolitik fermentlar faolligi aniqlandi.

Abstract

A total of 8 endophytic isolates were isolated from the root of the medicinal plant Kalanchoe degremona, 3 from the stem and 3 from the leaves. As a result of the screening, isolate K7, isolated from Kalanchoe degremona, was isolated and identified by MALDI TOF mass spectrometry. The isolated bacterial isolate K7 was identified as B. amyloliquefaciens and named B. amyloliquefaciens - strain UzMU 22. The antigonistic activity of the Bacillus amyloliquefaciens strain - UzMU 22 was determined against such pathogenic fungi as Verticillium dahliae, Alternaria alternata, Fusarium solani and Fusarium oxysporum, sensitivity to a number of antibiotics, such as kanamycin, erythromycin, cefotoxin, ceprofloxacin, gentamicin and lencomycin, as well as the activity of hydrolytic enzymes.

References

v1. Normurodova K.T., Nurmatov Sh.Kh., Alimova B.Kh., Akhmedova Z.R., Makhsumkhanov, A.A. Isolation and characteristics of highly active α-amylase from Bacillus subtilis-150 // Chemistry of Natural Compoundsthis, 2007, 43(4), P. 454–457. (Research Gate, IF- 0.33).
2. Normurodova K.T., MakhsumkhanovA.A., Alimova B.Kh., Pulatova O.M., Bozorov N.I. Isolation, purification and characterization of highly active protease from Bacillus subtilis-150 // Chemistry of Natural Compounds. –Vol. 46, №5. 2010. – Р. 833-834. (Research Gate, IF- 0.33).
3. Normurodova K.T., Abdusamatov S.А. Extraction of glucoamylase enzyme from Aspergillus oryzae UzMU K-14 fungal strain // “UzMU news” journal. - Tashkent, 2017. №3/2. – P. 114-116.
4. Нормуродова Қ.Т., Шохиддинова М.Н. Эндофит бактерияларининг қишлоқ хўжалигидаги истиқболлари // “ЎзМУ хабарлари” журнали. - Тошкент, 2020. №3/2. – Б. 108-112.
5. Чеботарь В.К., Заплаткин А.Н., Щербаков А.В., и др. Микробные препараты на основе эндофитных и ризобактерий, которые перспективны для повышения продуктивности и эффективности использования минеральных удобрений у ярового ячменя и овощных культур // Сельскохозяйственная биология. – 2016. – Т. 51 - № 3. – С. 335–342.
6. Normurodova K.T., Shoxiddinova М.N. Introduction of endophitic bacteria of plants and study of their morphological and cultural properties // The American Journal of Horticulture and Floriculture Research, 2021, 03 (12-02), P. 4-9. (Impact Factor – 5.731).
7. Shoxiddinova М.N., Tojeyev B.B., Tojiyeva M.B. Search and isolation of endophytic bacteria from medicinal plants and determination of their morphological and cultural properties // Eurasian Journal of Research, Development and Innovation, 2021, 3, P. 23-25. (Impact Factor – 7.892).
Published
2024-03-29
How to Cite
Moxichehra SHOXIDDINOVA, Mujassam AXMEDOVA, Nozima YUSUFDJANOVA, Behro’z TOJIYEV, Moxichehra PATTAYEVA, & Qunduz NORMURODOVA. (2024). ANTAGONIST AND ANTIBACTERIAL PROPERTIES OF THE BACTERIAL STRAIN BACILLUS AMYLOLIQUEFACIENS –UZMU 22. News of the NUUz, 3(3.1), 168-172. https://doi.org/10.69617/uzmu.v3i3.1.1754

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